flow cytometry staining buffer  (Thermo Fisher)

Journal: Scientific Reports

Article Title: Oxidative stress-mediated impairment of human trophoblast cell proliferation by zinc pyrithione exposure

doi: 10.1038/s41598-026-38895-9

Figure Lengend Snippet: Effects of ZPT on Apoptosis and Cell Cycle. ( A ) Representative flow cytometry scatter plots of Annexin V-FITC/PI staining after 72-hour treatment with ZPT. The four quadrants represent viable cells (Annexin V⁻/PI⁻), early apoptotic cells (Annexin V⁺/PI⁻), late apoptotic cells (Annexin V⁺/PI⁺), and necrotic cells (Annexin V⁻/PI⁺). ( B ) Representative flow cytometry histograms of PI staining showing DNA content after 72-hour ZPT treatment. ( C ) Quantitative analysis of late apoptosis rate. Data are from three independent experiments and expressed as mean ± SD. ** p < 0.01. ( D ) Quantitative analysis of cell cycle distribution. The percentages of cells in each phase (G0/G1, S, G2/M) are summarized. Data are from three independent experiments and expressed as mean ± SD. ns: not significant.

Article Snippet: The pellet was resuspended in ice-cold PBS, followed by the addition of 1 mL DNA staining solution and 10 μL permeabilization solution (Lianke Biotechnology, CCS012).

Techniques: Flow Cytometry, Staining

Journal: Research

Article Title: Novel Combination of Irreversible Electroporation and Allogenic Chimeric Antigen Receptor T-Cell Therapy Synergizes Therapeutic Outcomes in a Preclinical Human Pancreatic Cancer Mouse Model

doi: 10.34133/research.1105

Figure Lengend Snippet: Chimeric antigen receptor (CAR) target binding analysis following irreversible electroporation. (A) Viable and intact cells following electroporation are still available for cell membrane mesothelin (MSLN) binding, while necrotic cells experience a decrease in binding. (B) Flow cytometry gating to isolate single cells and plots of calcein AM versus mesothelin at 3 h following IRE delivery using 0 V/cm (control), 1,000 V/cm, and 2,000 V/cm. (C) Control using mesothelin-negative Jurkats. (D) Mesothelin expression in AsPC-1 cells compared to that in Jurkats. (E) Cell viability 3 h after electroporation at different electric field strengths; one-way analysis of variance (ANOVA) with Tukey’s posttest and correction; mean ± SD; n = 4. (F) Percent mesothelin (Mes) expression of high-viability and low-viability cell populations 3 h after electroporation; multiple 2-tailed t test; mean ± SD; n = 4. (G) Live (green) and dead (red) imaging at 3 h and 7 d after treatment; the scale bar is 1 mm. (H) Viable cell count at different electric fields after IRE and following recovery; one-way ANOVA with Tukey’s posttest and correction within each timepoint; mean ± SD; n = 4. (I) Mesothelin binding for recovered cells at day 7; one-way ANOVA with Tukey’s posttest and correction within each timepoint; mean ± SD; n = 4. ns, not significant; ** P < 0.01; *** P < 0.001; **** P < 0.0001. IL-2, interleukin-2; IL-15, interleukin-15; IFNγ, interferon-γ; FSC-H, forward scatter height; FSC-A, forward scatter area.

Article Snippet: Cell suspensions were centrifuged at 500 × g, with the liquid aspirated and resuspended in 500 μl of flow cytometry buffer (Thermo Fisher, 00-4222-26).

Techniques: Binding Assay, Electroporation, Membrane, Flow Cytometry, Control, Expressing, Imaging, Cell Characterization